Optimization of Remediation Systems using Our Expanding Suite of Molecular Tools
Date and Time: Thu, Sep 26, 2019 1:00 PM - 2:00 PM EDT
Register at: https://attendee.gotowebinar.com/register/8498291798537147916
Presenter: Phil Dennis, M.A.Sc., Principal Molecular Biologist, SiREM
Phil Dennis is a Principal Scientist at SiREM where he has been employed for over 18 years. Phil holds a Master of Applied Science in Civil Engineering from the University of Toronto and an Honors B.Sc. in Molecular Biology and Genetics from the University of Guelph. Phil currently directs molecular testing services and is innovation lead for SiREM’s research and development program. Phil is a lead author or coauthor on numerous peer-reviewed and conference papers and regularly presents at industry conferences and universities on the topics of environmental genetic testing and biodegradation
Characterization of microbial communities in soil, sediment and groundwater using molecular genetic tools is commonly used to assess natural attenuation, enhanced bioremediation and for ongoing site management. Molecular genetic testing is also increasingly used for determining the microbial composition of water treatment systems, assessing the presence of pathogens, and managing conditions such as microbially induced corrosion and biofouling.
As the number of identified microorganisms and functional genes continues to increase, the applications for molecular testing are amplified. For example, we now know Dehalogenimonas is capable of converting trans-DCE to vinyl chloride (VC) and VC to ethene. This means Dehalococcoides is no longer the only microbe documented to perform the final steps in complete reductive dechlorination! Furthermore, a growing range of molecular tests are available for once “exotic” microbial processes, such as anaerobic BTEX degradation, 1,4-dioxane degradation and anammox are now being used to monitor site remediation by these processes.
For whole community assessment, next generation sequencing (NGS) is revolutionizing our ability to characterize and monitor biological communities. Two major types of commercially available NGS are 16S rRNA amplicon sequencing (16S-NGS) and shotgun metagenomics. 16S-NGS provides microbial community characterization and integrating “functional analysis” into the data reporting provides an overview of the metabolic capabilities of the entire community, markedly increasing data interpretability.
The other major type of NGS, shotgun metagenomics, identifies the microbes, but also the functional genes and other biota that may influence remediation processes, including fungi, protozoans and viruses.
This presentation will provide examples of how molecular testing provides practical solutions, and insights using case studies including an MNA site, an enhanced bioremediation site, a leachate treatment system and in controlling biofouling in a water treatment system.
|Event End Date||09-26-2019|
|Registration Start Date||08-30-2019|